2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Melanoma Marker (MART-1 + Tyrosinase + gp100)

Melanoma Marker (MART-1 + Tyrosinase + gp100)

Mouse Monoclonal Antibody [Clone A103 + T311 + HMB45]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (A103 + T311 + HMB45).

img

Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker MAb (A103 + T311 + HMB45).

Product Details

Synonyms

Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; TYR; PMEL17

Positive Control

COLO-38 or SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant hMART-1 protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG2a, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

20-22kDa (doublet) (MART); 70-80kDa (Tyrosinase); 26-100kDa (gp100)

Specificity & Comments

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.
  2. Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.
  3. Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203.

Bioinformatics

NBT00702