Home >> Antibodies >> Melanoma Marker (MART-1 + Tyrosinase + gp100)

Melanoma Marker (MART-1 + Tyrosinase + gp100)

Mouse Monoclonal Antibody [Clone A103 + T311 + HMB45]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00

459.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (A103 + T311 + HMB45).

Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker MAb (A103 + T311 + HMB45).

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (A103 + T311 + HMB45).

Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker MAb (A103 + T311 + HMB45).

Product Details

Synonyms

Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; TYR; PMEL17

Positive Control

COLO-38 or SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant hMART-1 protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG2a, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

20-22kDa (doublet) (MART); 70-80kDa (Tyrosinase); 26-100kDa (gp100)

Specificity & Comments

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.