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Home >> Antibodies >> MDM2

MDM2

Mouse Monoclonal Antibody [Clone SMP14]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot Analysis of human HepG2 cell lysate using MDM2 Mouse Monoclonal Antibody (SMP14).

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SDS-PAGE Analysis Purified MDM2 Mouse Monoclonal Antibody (SMP14). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human breast carcinoma stained with MDM2 Mouse Monoclonal Antibody (SMP14).

Product Details

Synonyms

ACTFS; Double minute 2 protein; E3 ubiquitin-protein ligase; Hdm2; HDMX; MDM2; MDM2 oncogene E3 ubiquitin protein ligase; MDM2BP; Mouse Double Minute 2; MTBP; Murine Double Minute Chromosome 2; p53 Binding Protein Mdm2; p53-binding protein Mdm2

Positive Control

HepG2 cells. Breast Carcinoma.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide (aa 154-167) of human MDM2 protein.

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~90kDa

Specificity & Comments

MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53. It can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. Overexpression of MDM2 protein is detected in a variety of cancers.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Thway K, et al. Am J Surg Pathol. 2012 Mar;36(3):462-9.

Bioinformatics

  •  Entrez Gene ID: 4193
  •  Gene Symbol: MDM2
  •  SwissProt: Q00987
  •  Chromosome Location: 12q15
  •  Unigene: 484551
NBT01721