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MDM2

Mouse Monoclonal Antibody [Clone MDM2/7942]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human ovarian cancer stained with MDM2 Mouse Monoclonal Antibody (MDM2/7942). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified MDM2 Mouse Monoclonal Antibody (MDM2/7942). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human ovarian cancer stained with MDM2 Mouse Monoclonal Antibody (MDM2/7942). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified MDM2 Mouse Monoclonal Antibody (MDM2/7942). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

ACTFS; Double minute 2 protein; E3 ubiquitin-protein ligase; Hdm2; HDMX; MDM2; MDM2 oncogene E3 ubiquitin protein ligase; MDM2BP; Mouse Double Minute 2; MTBP; Murine Double Minute Chromosome 2; p53 Binding Protein Mdm2; p53-binding protein Mdm2

Positive Control

Human pancreas.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MDM2 protein fragment (around aa 1-300) (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2, kappa

Mol. Weight of Antigen

~90kDa

Specificity & Comments

MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53. It can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. Overexpression of MDM2 protein is detected in a variety of cancers.