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Home >> Antibodies >> MCM7 / CDC47 (Proliferation Marker)

MCM7 / CDC47 (Proliferation Marker)

Mouse Monoclonal Antibody [Clone SPM379]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Monoclonal Antibody (SPM379).

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Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Mouse Monoclonal Antibody (SPM379).

Product Details

Synonyms

CDC47; DNA replication licensing factor MCM7; MCM7 mini chromosome maintenance deficient 7; Minichromosome Maintenance 7; Mini chromosome maintenance protein 7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146

Positive Control

Exponentially growing any cultured human cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human hCDC47 protein

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

88kDa

Specificity & Comments

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Koonin, E.V. 1993. A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication. Nucleic Acids Res. 21: 2541-2547.

Bioinformatics

  •  Entrez Gene ID: 4176
  •  Gene Symbol: MCM7
  •  SwissProt: P33993
  •  Chromosome Location: 7q21.3-q22.1
  •  Unigene: 438720
NBT01545