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Home >> Antibodies >> Maltose Binding Protein / MBP-probe

Maltose Binding Protein / MBP-probe

Mouse Monoclonal Antibody [Clone R29.6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Maltose Binding Protein Mouse Monoclonal Antibody (R29.6). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of MBP-Tag recombinant protein using Maltose Binding Protein Mouse Monoclonal Antibody (R29.6).

Product Details

Synonyms

ECK4026; JW3994; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein

Positive Control

Saos-2 cells. MBP fusion proteins.

Known Applications & Suggested Dilutions

IF, Western Blot         More Details

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

MOS maltose binding protein fusion protein.

Cellular Localization

Periplasm.

Species Reactivity

MBP fusion proteins.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40kDa

Specificity & Comments

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western Blot Analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kamennaya NA et al. Appl Environ Microbiol 77:291-301 (2011).

Bioinformatics

NBT02847