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Home >> Antibodies >> Major Vault Protein (MVP)

Major Vault Protein (MVP)

Mouse Monoclonal Antibody [Clone 1014]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot Analysis of human HeLa cell lysate using Major Vault Protein (MVP) Mouse Monoclonal Antibody (Clone 1014).

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Immunofluorescence Analysis of A549 cells labeling MVP with MVP Monoclonal Antibody (Clone 1014) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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SDS-PAGE Analysis Purified MVP Monoclonal Antibody (Clone 1014). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Lung resistance-related protein (LRP); Major Vault Protein; MVP; VAULT1

Positive Control

MCF-7 or HeLa cells. Breast tumors.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

proteins precipitated from human breast cancer MCF-7 cells

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

104-110kDa

Specificity & Comments

Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Abbondanza C., et al. J Cell Biol 1998;141(6):1301-1310.
  2. Den Boer, M.L. et al. Blood 91, 2092-2098 (1998).

Bioinformatics

  •  Entrez Gene ID: 9961
  •  Gene Symbol: MVP
  •  SwissProt: Q14764
  •  Chromosome Location: 16p11.2
  •  Unigene: 632177
NBT00224