Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Printable Datasheet

Macrophage & Histiocytoma Marker Antibody [D11]

In Stock
Catalog Number Formulation Size Price
MSM2-348-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM2-348-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM2-348-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified Macrophage Monoclonal Antibody (D11). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Macrophage Monoclonal Antibody (D11). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Liver or histiocytoma.
Immunogen
Membrane preparation from human hepatocytes
Alternate Names
N/A, N/A, Not Known

Database Links

Entrez Gene ID
Not Known
SwissProt
N/A

Additional Information

Clone
D11
Mol. Weight of Antigen
125kDa -135kDa

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Rudinskaya TD, Poltoranina VS, Baranov VN, Petrovichev NN, Voytenkov BO, Tretyakov LO. D11, a novel monoclonal antibody specific for human mature macrophages and peripheral blood monocytes. Immunol Lett. 1992 Jun;33(1):1-7.
  • Frenkel MA, Tupitsyn NN, Rudinskaia TD, Poltoranina VS, Lebedeva NB, Volkova MA, Kaletin GI. [Use of a new antimacrophage monoclonal antibody D11 in diagnosis of hemoblastosis]. [Article in Russian] Gematol Transfuziol. 1995 Jul-Aug;40(4):13-6.
  • Tupitsyn NN, et al. Reactivity of anti-macrophage monoclonal antibody D11 in human leukemia and malignant lymphoma. Int J Cancer. 1996;68(2):160-3.
  • Petrovichev NN, et al. Antimacrophage monoclonal antibody D11 in the diagnosis of tumors of histiocytic origin. Acta Cytol. 1997;41(2):357-63. 2.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Macrophage & Histiocytoma Marker Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales