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Home >> Antibodies >> Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)

Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)

Mouse Monoclonal Antibody [Clone LAG3/3261]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified Monospecific Mouse Monoclonal Antibody to LAG-3 (LAG3/3261). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using LAG-3 Mouse Monoclonal Antibody (LAG3/3261).

Product Details

Synonyms

CD223; FDC protein; LAG-3

Positive Control

Human tonsil or Hodgkin's lymphoma (IHC-P).

Known Applications & Suggested Dilutions

ELISA         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human LAG3 protein

Cellular Localization

Membrane.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

70kDa

Specificity & Comments

LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4+CD8+ T cells and NK cell, with shared homology in structure to CD4 molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3+CD4+CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3+ cells) and inhibit induced (i.e. antigen-specific) Treg suppressive activity.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Andrews LP., et al. Immunol Rev. 2017 Mar;276(1):80-96. doi: 10.1111/imr.12519
  2. Demeure, C.E., et al. 2001. Eur. J. Cancer 37: 1709-1718

Bioinformatics

  •  Entrez Gene ID: 3902
  •  Gene Symbol: LAG3
  •  SwissProt: P18627
  •  Chromosome Location: 12p13.31
  •  Unigene: 409523
NBT03261