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Home >> Antibodies >> LEF1 / TCF1 alpha (Transcription Factor)

LEF1 / TCF1 alpha (Transcription Factor)

Mouse Monoclonal Antibody [Clone LEF1/6764]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human tonsil stained with LEF1 Mouse Monoclonal Antibody (LEF1/6764). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human tonsil stained with LEF1 Mouse Monoclonal Antibody (LEF1/6764). Inset: PBS instead of primary antibody; secondary only negative control.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using LEF1-Monospecific Mouse Monoclonal Antibody (LEF1/6764).

Product Details

Synonyms

Lymphoid enhancer-binding factor 1 (LEF1); T cell specific transcription factor 1 alpha (TCF1a); TCF1 alpha; TCF10; TCF7L3; Transcription factor T cell specific 1 alpha

Positive Control

Human tonsil and thymus.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human LEF1 protein (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

44kDa

Specificity & Comments

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ji Q, et al. Nat Commun 10:313 (2019).
  2. Przybyl J, et al. Gynecol Oncol 149:388-393 (2018).

Bioinformatics

  •  Entrez Gene ID: 51176
  •  Gene Symbol: LEF1
  •  SwissProt: Q9UJU2
  •  Chromosome Location: 4q25
  •  Unigene: 726506