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Home >> Antibodies >> Lambda Light Chain (B-Cell Marker)

Lambda Light Chain (B-Cell Marker)

Mouse Monoclonal Antibody [Clone HP6054]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF488

Price (USD)

429.00
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Confocal Immunofluorescent analysis of Ramos cells using CF488-labeled Lambda Light Chain Monoclonal Antibody (HP6054) (Green). DAPI was used to stain the cell nuclei (blue).

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Confocal Immunofluorescent analysis of Ramos cells using CF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Product Details

Synonyms

Bence Jones Protein; BJP; IGLC 1/2/3; Mcg Marker; Paraprotein

Positive Control

293T, Raji or hPBL cells. Tonsil or Spleen.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)
Optimal dilution for a specific application should be determined.

Immunogen

Purified human IgG myeloma proteins coupled to polyaminostyren microbeads

Cellular Localization

Cell Surface, cytoplasmic and secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

~22.5kDa

Specificity & Comments

This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

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Key References

  1. Reimer et al. Hybridoma 3: 263, 1984.

Bioinformatics

NBT00262