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Home >> Antibodies >> Lambda Light Chain (B-Cell Marker)

Lambda Light Chain (B-Cell Marker)

Mouse Monoclonal Antibody [Clone HP6054]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with Lambda Light Chain Mouse Monoclonal Antibody (HP6054).

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Western Blot analysis of human intestinal lysate using Lambda Light Chain Mouse Monoclonal Antibody (HP6054).

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Confocal Immunofluorescent analysis of Ramos cells using AF488-labeled Lambda Light Chain Monoclonal Antibody (HP6054) (Green). DAPI was used to stain the cell nuclei (blue).

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Confocal Immunofluorescent analysis of Ramos cells using AF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

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SDS-PAGE Analysis Purified Lambda Light Chain Mouse Monoclonal Antibody (HP6054). Confirmation of Integrity and Purity of Antibody

Product Details

Synonyms

Bence Jones Protein; BJP; IGLC 1/2/3; Mcg Marker; Paraprotein

Positive Control

293T, Raji or hPBL cells. Tonsil or Spleen.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Purified human IgG myeloma proteins coupled to polyaminostyren microbeads

Cellular Localization

Cell Surface, cytoplasmic and secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

~22.5kDa

Specificity & Comments

This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Reimer et al. Hybridoma 3: 263, 1984.

Bioinformatics

NBT00262