Flow Cytometric analysis of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku Monoclonal Antibody (KU729).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. MAb KU729 recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.