Immunofluorescent analysis of PFA-fixed HeLa cells. Ku Mouse Monoclonal Antibody (KU729) followed by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin (red).
Flow Cytometry of human Ku (p70/p80) on 293T cells. cells alone; Grey: isotype control; Green: AF488-labeled Ku Mouse Monoclonal Antibody (KU729).
Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: isotype control; Red: PE-labeled Ku Mouse Monoclonal Antibody (KU729).
SDS-PAGE Analysis Purified Ku Mouse Monoclonal Antibody (KU729) Confirmation of Purity and Integrity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.