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Home >> Antibodies >> Ku (p70/p80) (Nuclear Marker)

Ku (p70/p80) (Nuclear Marker)

Mouse Monoclonal Antibody [Clone KU729]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku Mouse Monoclonal Antibody (KU729).

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Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku Mouse Monoclonal Antibody (KU729).

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Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using Ku Mouse Monoclonal Antibody (KU729) followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.

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SDS-PAGE Analysis Purified Ku Mouse Monoclonal Antibody (KU729). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Ku (p70): 70kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70kDa subunit; CTC box-binding factor 75kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen, 70kDa; Ku70; Kup70; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80): 86kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 86Kd subunit; ATP-dependent DNA helicase II 80kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 5 (XRCC5)

Positive Control

Human cancer K562, HL-60, HEp-2 or HeLa cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, ICC         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Immunogen

Nuclear extract of human HL-60 cells

Cellular Localization

Nuclear

Species Reactivity

Human. Primates. Does not react with Mouse, Rat or Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70kDa & 80kDa

Specificity & Comments

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Walker, J. R., et al. 2001. Nature 412:607.

Bioinformatics

NBT00729