Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku Mouse Monoclonal Antibody (KU729).
Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku Mouse Monoclonal Antibody (KU729).
Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using Ku Mouse Monoclonal Antibody (KU729) followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.
SDS-PAGE Analysis Purified Ku Mouse Monoclonal Antibody (KU729). Confirmation of Integrity and Purity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.