2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> KBA.62 (Melanoma Associated Antigen)

KBA.62 (Melanoma Associated Antigen)

Mouse Monoclonal Antibody [Clone KBA.62]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Monoclonal Antibody (KBA.62).

img

Formalin-fixed, paraffin-embedded human Tongue stained with Melanoma Monoclonal Antibody (KBA.62).

img

SDS-PAGE Analysis Purified Melanoma Monoclonal Antibody (KBA.62). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Human Melanoma Associated Antigen; KBA.62

Positive Control

SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Applications & Suggested Dilutions

IF, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human KAL cells derived from lymph node metastasis of malignant melanoma

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Multiple (140, 135 and 128kDa and two weak bands of 88 and 73kDa)

Specificity & Comments

KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. E Cohen-Knafo, T al Saati, J Aziza, E Ralfkiaer, J Selves, B Gorguet, and G Delsol. Production and characterisation of an antimelanoma monoclonal antibody KBA.62 using a new melanoma cell line reactive on paraffin wax embedded sections. J Clin Pathol. 1995; 48(9): 826-831.
  2. Cecile Pages et. al. KBA.62: a useful marker for primary and metastatic melanomas. Human Pathology 39(8):1136-1142; 2008.

Bioinformatics

NBT00895