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Home >> Antibodies >> Kappa Light Chain (B-Cell Marker)

Kappa Light Chain (B-Cell Marker)

Mouse Monoclonal Antibody [Clone Kap-56]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Monoclonal Antibody (Kap-56).

Product Details

Synonyms

HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM

Positive Control

293T, Raji or hPBL cells. Tonsil or Spleen.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (0.5-2.0ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human lymphocytes stimulated

Cellular Localization

Cell Surface, cytoplasmic and secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~22.5kDa

Specificity & Comments

This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kiyotaki M et. al. J Immunol. 1987;138(12):4150-8.
  2. Nakamura T et. al. Proc Natl Acad Sci U S A. 1992;89(18):8522-6.

Bioinformatics

  •  Entrez Gene ID: 3514
  •  Gene Symbol: IGKC
  •  SwissProt: P01601, P01834
  •  Chromosome Location: 2p11.2
  •  Unigene: 449609
NBT00859