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Home >> Antibodies >> Involucrin (Squamous Cell Terminal Differentiation Marker)

Involucrin (Squamous Cell Terminal Differentiation Marker)

Mouse Monoclonal Antibody [Clone IVRN/827]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with Involucrin Monoclonal Antibody (IVRN/827)

Product Details

Synonyms

IVL

Positive Control

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37'C)(Note than enzyme digestion is better than citrate buffer based epitope unmasking)
Optimal dilution for a specific application should be determined.

Immunogen

Purified involucrin from human keratinocytes

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

66-170kDa

Specificity & Comments

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Eckert, R.L. and Green, H. 1986. Structure and evolution of the human involucrin gene. Cell 46: 583-589.

Bioinformatics

  •  Entrez Gene ID: 3713
  •  Gene Symbol: IVL
  •  SwissProt: P07476
  •  Chromosome Location: 1q21.3
  •  Unigene: 516439
NBT00827