Formalin-fixed, paraffin-embedded human colon carcinomastained with Interleukin-7 (IL-7) Mouse Monoclonal Antibody (IL7/4013). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis Purified IL-7Mouse Monoclonal Antibody (IL7/4013). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed U-87 cells stained using Interleukin-7 (IL-7) Mouse Monoclonal Antibody (IL7/4013) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow cytometric analysis of PFA-fixed U-87 cells. Interleukin-7 (IL-7) Mouse Monoclonal Antibody (IL7/4013) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Analysis of Protein Array containing >19,000 full-length human proteins using Interleukin-7 (IL-7) Monospecific Mouse Monoclonal Antibody (IL7/4013).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Interleukin-7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor specific chain and the IL-2 receptor chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of Insulin receptor substrate-1 (IRS-1) and Insulin receptor substrate-2 (IRS-2), originally called 4PS.