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Home >> Antibodies >> Interferon alpha-2 (IFNA2)

Interferon alpha-2 (IFNA2)

Mouse Monoclonal Antibody [Clone N27]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified IFNA2 Mouse Monoclonal Antibody (N27). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Alpha 2a interferon; IFN alpha; IFN-alpha-2; IFNA; IFNA2; IFNA2B; Interferon alpha 2a; Interferon alpha 2b; Interferon alpha-2; Interferon alpha-A; LeIF2; LeIFA

Positive Control

Stimulated human peripheral blood mononuclear cells.

Known Applications & Suggested Dilutions

ELISA, BL, Flow Cyt, IF         More Details

ELISA (For coating, order Ab without BSA)
Blocking Activity (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Purified recombinant human IFN-alpha2

Cellular Localization

Cytoplasmic & Extracellular (Secreted)

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

16-27kDa

Specificity & Comments

Recognizes a protein of 16-27kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This MAb is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this MAb is called site I and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 MAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Aman MJ et al. Interferon-alpha stimulates production of interleukin-10 in activated CD4+ T cells and monocytes. Blood 1996, 87(11):4731-4736
  2. Kontsek P et al. Mapping of two immunodominant structures on human interferon alpha 2c and their role in binding to cells. Mol Immunol 1991, 28:1289-1297
  3. Pestka S et al. Interferons and their actions. Annu Rev Biochem 1987, 56:727-777
  4. Kontsek P et al. Peptide-mapping of three neutralizing epitopes into predicted biologically active sites of human interferon-alpha 2. Immunol Lett 1993, 35(3):281-284
  5. Capon DJ et al. Two distinct families of human and bovine interferon-alpha genes are coordinately expressed and encode functional polypeptides. Mol Cell Biol 1985, 5(4):768-779
  6. Sen GC et al.The interferon system. A bird's eye view of its biochemistry. J Biol Chem 1992, 267(8):5017-5020
  7. Kurane I et al. Induction of interferon alpha from human lymphocytes by autologous, dengue virus-infected monocytes. J Exp Med 1987, 166(4):999-1010
  8. Lepe-Zuniga JL et al. Production of interferon-alpha induced by dsRNA in human peripheral blood mononuclear cell cultures: role of priming by dsRNA-induced interferons-gamma and -beta. J Interferon Res 1989, 9(4):445-456

Bioinformatics

  •  Entrez Gene ID: 3440
  •  Gene Symbol: IFNA2
  •  SwissProt: P01563
  •  Chromosome Location: 9q22
  •  Unigene: 211575
NBT00330