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Home >> Antibodies >> IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker)

IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker)

Recombinant Mouse Monoclonal Antibody [Clone rIGHM/1623]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified IgM Recombinant Mouse Monoclonal Antibody (rIGHM/1623). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human tonsil stained with IgM Recombinant Mouse Monoclonal Antibody (rIGHM/1623).

Product Details

Synonyms

AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region

Positive Control

293T, Raji or hPBL cells. Human tonsil or spleen (IHC).

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length IGHM protein

Cellular Localization

Cytoplasm, Cell Surface and Secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

50-75kDa

Specificity & Comments

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Baryshnikov AIu. Gematol Transfuziol. 1990 Aug;35(8):4-7.
  2. Martinova T.et al., In: Problems medical biotechnology and immunological infection diseases. Vol 11, 182-186, 19

Bioinformatics

  •  Entrez Gene ID: 3507
  •  Gene Symbol: IGHM
  •  SwissProt: P01871, P20769
  •  Chromosome Location: 14q32.33
  •  Unigene: 510635
NBT03804