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Home >> Antibodies >> IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker)

IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone IGHD/8228R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human tonsil stained with IgD Recombinant Rabbit Monoclonal Antibody (IGHD/8228R). Inset: PBS instead of primary antibody; secondary only negative control.

img

Formalin-fixed, paraffin-embedded human tonsil stained with IgD Recombinant Rabbit Monoclonal Antibody (IGHD/8228R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

Ig delta chain C region; IGHD; Immunoglobulin heavy constant delta

Positive Control

Human tonsil lysate or NAMALWA cell lysate. Human tonsil and lymphoid tissues

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa1-200) of human IGHD protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

44-80kDa (heavy chain); 21-25kDa (kappa light chain)

Specificity & Comments

Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin D (IgD) exists as a monomer with δ heavy chains and either κ or λ light chains. It plays a biological role as a transmembrane receptor molecule, co-expressed with IgM on the surface of mature/naive B cells. In particular, it is found on spleen B cell surfaces. Compared to IgM, IgD exists in much lower numbers and is not expressed on immature B cells. IgD surface expression on B cells is regulated in part by IL-27. In mice, the inhibition of this immunoglobulin isotype does not cause a significant change to the immune system.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Takayasu, T., et al. 1980. Biophys. Res. Commun. 97: 635-641.

Bioinformatics

  •  Entrez Gene ID: 3495
  •  Gene Symbol: IGHD
  •  SwissProt: P01880
  •  Chromosome Location: 14q13
  •  Unigene: 510635