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Home >> Antibodies >> IgA (Immunoglobulin Alpha Heavy Chain) (B-Cell Marker)

IgA (Immunoglobulin Alpha Heavy Chain) (B-Cell Marker)

Mouse Monoclonal Antibody [Clone SPM187]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00

Product Details

Synonyms

A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; IGHA1; IGHA2; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin Heavy Constant Alpha 1; Immunoglobulin Heavy Constant Alpha 2

Positive Control

Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml 30 min RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml 30 min RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Purified human IgA

Cellular Localization

Cytoplasm, Cell Surface and Secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

50-75kDa

Specificity & Comments

This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain. It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkins lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Biewenga J. et al. Domain specificity and assay specificity of monoclonal antibodies against human IgA. Adv. Exp. Med. Biol. 216B: 1239 1249 (1987).
  2. Biewenga J. et al. Monoclonal antibodies against different domains of human IgA: Specificities determined by immunoblotting and haemagglutinationinhibition. Mol. Immunol. 23: 761 767 (1986).
  3. Mestecky J. et al. Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study. J. Immunol. Meth. 193: 103 148 (1996).

Bioinformatics

NBT00148