Home >> Antibodies >> IDH1-R132H (Isocitrate Dehydrogenase)

IDH1-R132H (Isocitrate Dehydrogenase)

Recombinant Rabbit Monoclonal Antibody [Clone IDH1.R132H/7328R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

FFPE human prostate with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit MAb (IDH1.R132H/7328R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

FFPE human brain with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit MAb (IDH1.R132H/7328R). Inset: PBS instead of primary antibody; secondary only negative control.

FFPE human prostate with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit MAb (IDH1.R132H/7328R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

FFPE human brain with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit MAb (IDH1.R132H/7328R). Inset: PBS instead of primary antibody; secondary only negative control.

Product Details

Synonyms

Cytosolic NADP-isocitrate dehydrogenase; Epididymis luminal protein 216; Epididymis secretory protein Li 26; HEL-216; HEL-S-26; ICDH; IDCD; IDH1; IDP; IDPC; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP dependent isocitrate dehydrogenase peroxisomal; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; PICD

Positive Control

HepG2, HeLa, HT29 or MCF7 cells. Human breast, colon or prostate carcinoma.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 1-200) of human IDH1 (exact sequence is proprietary)

Cellular Localization

Cytoplasm. Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

45-47kDa

Specificity & Comments

IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.