2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Human Nucleolar Antigen (Marker For Human Cells)

Human Nucleolar Antigen (Marker For Human Cells)

Mouse Monoclonal Antibody [Clone NM95]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF488

Price (USD)

429.00
img

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CF488-labeled human Nucleolar Monoclonal Antibody (NM95).

img

Flow Cytometric Analysis of human Nucleolar Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled human Nucleolar Monoclonal Antibody (NM95).

Product Details

Synonyms

Not Known

Positive Control

Any human cells. Tissues.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of myeloid leukemia biopsy cells

Cellular Localization

Nucleoli

Species Reactivity

Human. Does not react with Mouse, Rat or Cow.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Not Known

Specificity & Comments

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Related Products



Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.

Bioinformatics

NBT00095