2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Human Nucleolar Antigen (Marker For Human Cells)

Human Nucleolar Antigen (Marker For Human Cells)

Mouse Monoclonal Antibody [Clone NM95]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nucleolar Mouse Monoclonal Antibody (NM95).

img

Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolar Mouse Monoclonal Antibody (NM95).

img

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with AF488 Conjugate of Nucleolar Mouse Monoclonal Antibody (NM95).

img

Flow Cytometry of Human Nucleolar Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled human Nucleolar MAb (NM95).

img

SDS-PAGE Analysis Purified Nucleolar Mouse Monoclonal Antibody (NM95). Confirmation of Purity and Integrity of Antibody

img

Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

img

Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.

Product Details

Synonyms

Not Known

Positive Control

Any human cells. Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, ICC, IHC-P         More Details

Flow Cytometry (1-2ug/million cells
Immunofluorescence (1-2ug/ml)
Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of myeloid leukemia biopsy cells

Cellular Localization

Nucleoli

Species Reactivity

Human. Does not react with Mouse, Rat or Cow.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Not Known

Specificity & Comments

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.

Bioinformatics

NBT00095