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Home >> Antibodies >> Human Nuclear Antigen (HNA) (Human CellMarker)

Human Nuclear Antigen (HNA) (Human CellMarker)

Recombinant Rabbit Monoclonal Antibody [Clone 235-1R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Immunofluorescence Analysis of PFA-fixed HeLa cells with Hu Nuclear Antigen Recombinant Rabbit Monoclonal Antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green).

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Immunofluorescence Analysis of MeOH-fixed HeLa cells with Hu Nuclear Antigen Recombinant Rabbit Monoclonal Antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green).

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SDS-PAGE Analysis Purified HNA Recombinant Rabbit Monoclonal Antibody (235-1R). Confirmation of Integrity and Purity of the Antibody.

Product Details

Synonyms

HNA; HuNu

Positive Control

All human cells. Human tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IP, ICC         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunoprecipitation (1-2 g/500ug protein lysate)
Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT),Immunohistology (Frozen) (1-2ug/ml for 30 minutes at RT)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Cellular Localization

Nuclei

Species Reactivity

Human and Non-human Primates. Does not react with mouse, rat and chicken.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

70kDa & 80kDa

Specificity & Comments

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Rosario Sanchez-Pernaute et. al. Brain. 2008;131(8): 2127 2139.
  2. Glaser R, et al. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease

Bioinformatics