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Home >> Antibodies >> Human Nuclear Antigen (HNA) (Human Cell Marker)

Human Nuclear Antigen (HNA) (Human Cell Marker)

Mouse Monoclonal Antibody [Clone 235-1]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to PE

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to PE

Price (USD)

429.00
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Flow Cytometric analysis of Human Nuclear Antigen on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).

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Flow Cytometric analysis of Human Nuclear Antigen on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).

Product Details

Synonyms

HNA; HuNu

Positive Control

All human cells. Tissues.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100 for 30 minutes at RT)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Cellular Localization

Nuclei

Species Reactivity

Human. Non-Human primates. Does not react with Mouse, Rat or Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70kDa & 80kDa

Specificity & Comments

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127-2139.
  2. Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease

Bioinformatics

NBT00099