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Home >> Antibodies >> Human Nuclear Antigen (HNA) (Human Cell Marker)

Human Nuclear Antigen (HNA) (Human Cell Marker)

Mouse Monoclonal Antibody [Clone 235-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Green). Counter stained with Phalloidin (red).

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Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Acetone-fixed frozen human Tonsil stained with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1).

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SDS-PAGE Analysis Purified Human Nuclear Antigen Mouse Monoclonal Antibody (235-1). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of PFA-fixed Jurkat cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

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Flow Cytometric Analysis of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

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Flow Cytometric Analysis of PFA-fixed MCF-7 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescent staining of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).

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Immunofluorescent staining of PFA-fixed MCF-7 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).

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Flow Cytometric Analysis of PFA-fixed Raji cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

HNA; HuNu

Positive Control

All human cells. Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IP, ICC, IHC-Fr         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunoprecipitation (1-2ug/500ug protein lysate)
Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT)
Immunohistochemistry (Frozen) (1-2ug/ml for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Cellular Localization

Nuclei

Species Reactivity

Human. Non-Human primates. Does not react with Mouse, Rat or Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70kDa & 80kDa

Specificity & Comments

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127-2139.
  2. Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease

Bioinformatics

NBT00099