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Home >> Antibodies >> HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Mouse Monoclonal Antibody [Clone SPM253]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with HSP60 Monoclonal Antibody (SPM253)

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Formalin-fixed, paraffin-embedded human Pancreas stained with HSP60 Monoclonal Antibody (SPM253)

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Immunofluorescence Analysis of PFA-fixed MCF-7 cells. HSP60 Mouse Monoclonal Antibody (SPM253) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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Western Blot Analysis of HeLa cell lysate using HSP60 Mouse Monoclonal Antibody (SPM253)

Product Details

Synonyms

60kDa chaperonin, 60kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)

Positive Control

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human HSP60 protein

Cellular Localization

Mitochondria in cytoplasm

Species Reactivity

Human. Mouse. Rat. Hamster. Sheep. Rabbit. Cow. Dog. Pig. Monkey. Chicken. Xenopus laevis. Drosophila. Does not react with bacteria, helminths or spinach.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Boog CJ; de Graeff-Meeder ER; Lucassen MA; van der Zee R; Voorhorst-Ogink MM; van Kooten PJ; Geuze HJ; van Eden W. Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis. Journal of Experimental Medicine, 1992, 175(6):1805-10.

Bioinformatics

  •  Entrez Gene ID: 3329
  •  Gene Symbol: HSPD1
  •  SwissProt: P10809
  •  Chromosome Location: 2q33.1
  •  Unigene: 595053
NBT00851