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Home >> Antibodies >> HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Mouse Monoclonal Antibody [Clone LK2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Immunofluorescence staining of MEOH-fixed HeLa cells using HSP60 Mouse Monoclonal Antibody (LK2) followed by goat anti-mouse IgG-CF488 (green); nuclei labeled with NucSpot (red).

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SDS-PAGE Analysis Purified HSP60 Mouse Monoclonal Antibody (LK2) Confirmation of Integrity and Purity of Antibody.

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Western blot analysis of Hep2 cell lysate using HSP60 Mouse Monoclonal Antibody (LK2).

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Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (LK2).

Product Details

Synonyms

60kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Spastic paraplegia 13 (SPG13)

Positive Control

HeLa, Hep2 or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Known Applications & Suggested Dilutions

IF, Western Blot, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human HSP60 protein

Cellular Localization

Mitochondrial

Species Reactivity

Human, Monkey, Mouse, Rat, Hamster, Guinea pig, Chicken, E. coli, Borellia, Streptococcus pyogenes, Yersinia entercolitica, Salmonella typhimurium, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Helminths, Yeast, and Spinach.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-419 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Boog CJ et al. Journal of Experimental Medicine, 1992, 175(6):1805-10.

Bioinformatics

  •  Entrez Gene ID: 3329
  •  Gene Symbol: HSPD1
  •  SwissProt: P10809
  •  Chromosome Location: 2q33.1
  •  Unigene: 595053