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Home >> Antibodies >> HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Mouse Monoclonal Antibody [Clone GROEL/730]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with HSP60 Monoclonal Antibody (GROEL/730)

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Formalin-fixed, paraffin-embedded human Tonsil stained with HSP60 Monoclonal Antibody (GROEL/730

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Formalin-fixed, paraffin-embedded human Pancreas stained with HSP60 Monoclonal Antibody (GROEL/730).

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Western Blot Analysis of HeLa cell lysate using HSP60 Monoclonal Antibody (GROEL/730).

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Confocal immunofluorescence image of HeLa cells using HSP60 Monoclonal Antibody (GROEL/730). Green (CF488) and Reddot is used to label the nuclei Red.

Product Details

Synonyms

60kDa chaperonin, 60kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)

Positive Control

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human HSP60 protein

Cellular Localization

Mitochondria in cytoplasm

Species Reactivity

Human specific. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone GROEL730 reacts only with human and is useful in distinguishing human hsp60 from other mammalian and bacterial hsp60.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Schlesinger, M.J., et al. 1982. Heat Shock: from Bacteria to Man. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory.

Bioinformatics

  •  Entrez Gene ID: 3329
  •  Gene Symbol: HSPD1
  •  SwissProt: P10809
  •  Chromosome Location: 2q33.1
  •  Unigene: 595053
NBT00730