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Home >> Antibodies >> HSP27 (Heat Shock Protein 27)

HSP27 (Heat Shock Protein 27)

Mouse Monoclonal Antibody [Clone SPM252]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).

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Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).

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Western blot analysis of HeLa cell lysate using HSP27 Mouse Monoclonal Antibody (SPM252).

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SDS-PAGE Analysis Purified HSP27 Mouse Monoclonal Antibody (SPM252). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27. HSP27 Mouse Monoclonal Antibody (SPM252) followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is RedDot.

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Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Mouse Monoclonal Antibody (SPM252) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Positive Control

MCF-7 or HeLa cells. ~50% of breast carcinomas are positive for hsp27, especially those that are positive for ER and/or PgR.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Cellular Localization

Predominantly cytoplasmic with some nuclear

Species Reactivity

Human, Chimpanzee, Sheep, Rat, Mouseand Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

27kDa

Specificity & Comments

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Edwards DP et. al.BiochemBiophys Research Commun, 93:804-812, 1980.
  2. Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 1991.

Bioinformatics

  •  Entrez Gene ID: 3315
  •  Gene Symbol: HSPB1
  •  SwissProt: P04792
  •  Chromosome Location: 7q11.23
  •  Unigene: 520973