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Home >> Antibodies >> HLA-Pan (MHC II)

HLA-Pan (MHC II)

Recombinant Rabbit Monoclonal Antibody [Clone HLA-Pan/2967R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-Pan Rabbit Recombinant Monoclonal Antibody (HLA-Pan/2967R).

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Flow Cytometric Analysis of Raji cells using HLA-Pan Recombinant Rabbit Monoclonal Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype control (Red).

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Flow Cytometric Analysis of Ramos cells using HLA-Pan Recombinant Rabbit Monoclonal Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype control (Red).

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Immunofluorescence staining of Raji cells using HLA-Pan Recombinant Rabbit Monoclonal Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with Reddot (Red).

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Western Blot Analysis of Ramos cell lysate using HLA-Pan Recombinant Rabbit Monoclonal Antibody (HLA-Pan/2967R).

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SDS-PAGE Analysis Purified HLA-Pan Rabbit Recombinant Monoclonal (HLA-Pan/2967R). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA-Pan Rabbit Recombinant Monoclonal Antibody (HLA-Pan/2967R).

Product Details

Synonyms

HLA-DPB1; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5

Positive Control

Ramos or Raji cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, Western Blot, IHC-P         More Details

ELISA (For coating use Ab at 2-5ug/ml, order Ab without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

33-35kDa

Specificity & Comments

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gatter et al. 1982. J Clin Pathol. 35(11):1253-67.
  2. Gatter et al. 1982. Semin Oncol. 9(4):517-25.

Bioinformatics

NBT02967