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Home >> Antibodies >> HLA-DRB (MHC II)

HLA-DRB (MHC II)

Mouse Monoclonal Antibody [Clone SPM289]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DR Monoclonal Antibody (SPM289).

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Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA-DR Monoclonal Antibody (SPM289).

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Immunofluorescence Analysis of Raji cells labeling HLA-DR with HLA-DR Monoclonal Antibody (SPM289) conjugated with APC (Green) The nuclear counterstain is Reddot (Red)

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Flow Cytometric Analysis of human Raji cells using HLA-DR Monoclonal Antibody (SPM289) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

DR alpha chain; DRB1; DRB4; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR alpha chain; HLA DR1B; HLA DR3B; HLA DRA; HLA DRA1; HLA DRB1; HLA DRB3; HLA DRB4; HLA DRB5; HLA-DRA; HLADR4B; HLADRA1; HLADRB; Major histocompatibility complex class II DR alpha; Major histocompatibility complex class II DR beta 1; Major histocompatibility complex class II DR beta 3; Major histocompatibility complex class II DR beta 4; Major histocompatibility complex class II DR beta 5; MHC cell surface glycoprotein; MHC class II antigen DRA

Positive Control

Raji, Ramos, Daudi or HuT78 cells. Spleen, Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (2-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Activated human peripheral blood mononuclear cells

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

~28kDa (beta chain)

Specificity & Comments

This MAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Johnson MD; Glick AD; Davis BW. Immunohistochemical evaluation of Leu-7, myelin basic-protein, S100-protein, glial-fibrillary acidic-protein, and LN3 immunoreactivity in nerve sheath tumors and sarcomas. Archives of Pathology and Laboratory Medicine, 1988, 112(2):155-60.

Bioinformatics

  •  Entrez Gene ID: 3123
  •  Gene Symbol: HLA-DR
  •  SwissProt: P01903
  •  Chromosome Location: 6p21.3
  •  Unigene: 520048
NBT00057