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Home >> Antibodies >> HLA-DRB (MHC II)

HLA-DRB (MHC II)

Mouse Monoclonal Antibody [Clone L243]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified HLA-DRB Mouse Monoclonal Antibody (L243). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

DRB1; HLA class II histocompatibility antigen, DR-1 beta chain; HLA-DR-beta 1; HLA-DRB1; human leucocyte antigen DRB1; Leucocyte antigen DR beta 1 chain; lymphocyte antigen DRB1; major histocompatibility complex, class II, DR beta 1; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein

Positive Control

Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Activated human peripheral blood mononuclear cells

Cellular Localization

Cell Surface

Species Reactivity

Human. Baboon. Chimpanzee. Monkey. Squirrel. Dog.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

~28kDa (beta chain)

Specificity & Comments

This MAb reacts with the HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. It binds a conformational epitope on HLA-DR, which depends on the correct folding of the heterodimer. This MAb has been reported to block mixed lymphocyte reactions. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kD alpha (heavy) chain and a 28kD beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. Immunogenetics. 1984;19(3):179-94
  2. Horejsi, V., et al. 1986. Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens 28: 288-297.
  3. Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc Natl Acad Sci U S A. 1981;78(3):1791-5.

Bioinformatics

  •  Entrez Gene ID: 3123
  •  Gene Symbol: HLA-DRB1
  •  SwissProt: P01911
  •  Chromosome Location: 6p21.3
  •  Unigene: 534322
NBT00458