Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DR Monoclonal Antibody (HLA-DRB/7795R). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis Purified HLA-DR Monoclonal Antibody (HLA-DRB/7795R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human lymph node stained with HLA-DR Monoclonal Antibody (HLA-DRB/7795R). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Western blot analysis of HDLM-2 cell lysate using HLA-DR Monoclonal Antibody (HLA-DRB/7795R).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.