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Home >> Antibodies >> HLA-DRA (MHC II)

HLA-DRA (MHC II)

Mouse Monoclonal Antibody [Clone 19-26.1; same as MB-26.1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DRA Mouse Monoclonal Antibody (19-26.1).

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Flow Cytometric Analysis of Raji cells. HLA-DRA Monoclonal Antibody (19-26.1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

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SDS-PAGE Analysis Purified HLA-DRA Monoclonal Antibody (19-26.1). Confirmation of Integrity and Purity of Antibody

Product Details

Synonyms

DR alpha chain; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR alpha chain; HLA DRA; HLA DRA1; HLA-DRA; HLADRA1; Major histocompatibility complex class II DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA

Positive Control

Raji, Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Raji cells

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

36kDa (α chain) and 27kDa (β chain)

Specificity & Comments

This MAb reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Thompson C et al. Hum Immunol 1983, 6(3):133-50
  2. Rask L Autoimmunity 1991, 8(3):237-244.

Bioinformatics

  •  Entrez Gene ID: 3122
  •  Gene Symbol: HLA-DRA
  •  SwissProt: P01903
  •  Chromosome Location: 6p21.3
  •  Unigene: 520048
NBT01082