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Home >> Antibodies >> HLA-DRA (MHC II)

HLA-DRA (MHC II)

Mouse Monoclonal Antibody [Clone 169-1B5.2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified HLA-DRA Mouse Monoclonal Antibody (169-1B5.2). Confirmation of Integrity and Purity of Antibody.

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Flow Cytometric Analysis of PFA-fixed Raji cells. HLA-DRA Mouse Monoclonal Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

DR alpha chain; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR alpha chain; HLA DRA; HLA DRA1; HLA-DRA; HLADRA1; Major histocompatibility complex class II DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA

Positive Control

Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Human peripheral blood leukocytes

Cellular Localization

Cell surface

Species Reactivity

Human. Cow. Cat.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

36kDa (α chain) and 27kDa (β chain)

Specificity & Comments

This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen.It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Vaughan J. et al. (1982): Proceedings of the second Asia and Oceania histocompatibility workshop conference. Melbourne. p221.
  2. Kuramochi T. et al. (1987): Cross-reactivity between human and feline Ia antigens, using a monoclonal antibody HLA-D.m1. Amer. J. Vet. Res. 48:186-188.

Bioinformatics

  •  Entrez Gene ID: 3122
  •  Gene Symbol: HLA-DRA
  •  SwissProt: P01903
  •  Chromosome Location: 6p21.3
  •  Unigene: 520048
NBT01096