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Home >> Antibodies >> HLA-DR (MHC II)

HLA-DR (MHC II)

Mouse Monoclonal Antibody [Clone TAL 1B5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Lung Tumor stained with HLA-DR Mouse Monoclonal Antibody (TAL 1B5).

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Flow Cytometric Analysis of Raji cells. HLA-DR Mouse Monoclonal Antibody (TAL 1B5) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HLA-DR Mouse Monoclonal Antibody (TAL 1B5).

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SDS-PAGE Analysis Purified HLA-DR Mouse Monoclonal Antibody (TAL 1B5). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of Ramos cell lysates using HLA-DR Mouse Monoclonal Antibody (TAL 1B5).

Product Details

Synonyms

DR alpha chain; DRB1; DRB4; HLA class II histocompatibility antigen DR alpha chain; HLA DR1B; HLA DR3B; HLA DRA; HLA-DRA; HLADR4B; HLADRA1; HLADRB; Major histocompatibility complex class II DR alpha; Major histocompatibility complex class II DR beta 1/3/4/5

Positive Control

Raji, Ramos or Daudi whole cell lysates. Spleen, tonsil or liver tissue lysates. Human melanoma, tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

36kDa (alpha chain); 27kDa (beta chain); 63kDa (HLA-DR dimer)

Specificity & Comments

Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC class II/ dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the sub- sequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain ( -Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Podolin, P.L., et al. 2008. J. Immunol. 180: 7989-8003.

Bioinformatics

  •  Entrez Gene ID: 3122
  •  Gene Symbol: HLA-DR
  •  SwissProt: P01903
  •  Chromosome Location: 6p21.3
  •  Unigene: 520048
NBT02187