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HLA-DR (MHC II)

Recombinant Rabbit Monoclonal Antibody [Clone HLA-DRA/6840R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DR Recombinant Rabbit Monoclonal Antibody (HLA-DRA/6840R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DR Recombinant Rabbit Monoclonal Antibody (HLA-DRA/6840R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified HLA-DR Recombinant Rabbit Monoclonal (HLA-DRA/6840R). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DR Recombinant Rabbit Monoclonal Antibody (HLA-DRA/6840R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DR Recombinant Rabbit Monoclonal Antibody (HLA-DRA/6840R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified HLA-DR Recombinant Rabbit Monoclonal (HLA-DRA/6840R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

DRB1; DRB4; HLA class II histocompatibility antigen DR alpha chain; HLA DR1B; HLA DR3B; HLA DRA; HLA DRA1; HLA DRB1; HLA DRB3; HLA DRB4; HLA DRB5; HLA-DRA; HLADR4B; HLADRA1; HLADRB; Major histocompatibility complex class II DR beta 1 / 3/ 4; Major histocompatibility complex class II DR beta 5; MHC cell surface glycoprotein

Positive Control

Human tonsil or lymph node.

Known Applications & Suggested Dilutions

IHC More Details

Immunohistochemistry (Frozen & Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa1-200) of human HLA-DR protein (exact sequence is proprietary)

Cellular Localization

Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

~28kDa (beta chain)

Specificity & Comments

This MAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. �It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the HLA-DRA/6840R monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.