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Home >> Antibodies >> Histone H1 (Pan Nuclear Marker)

Histone H1 (Pan Nuclear Marker)

Mouse Monoclonal Antibody [Clone SPM256]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with Histone H1 Mouse Monoclonal Antibody (SPM256).

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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Monoclonal Antibody (SPM256).

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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Monoclonal Antibody (SPM256)

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Confocal Immunofluorescence image of HeLa cells using Histone H1 Mouse Monoclonal Antibody (SPM256) (green-CF488). Phalloidin is used to label the membranes.

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Flow Cytometric Analysis of HeLa cells. Histone H1 Monoclonal Antibody (SPM256) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

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Western Blot Analysis of human heart tissue lysate using Histone H1 Mouse Monoclonal Antibody (SPM256).

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SDS-PAGE Analysis Purified Histone H1 Monoclonal Antibody (SPM256). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

H1(0); H1.1; H1.2; H1.3; H1.4; H1.5; H10; H1A; H1F0; H1F1; H1F2; H1F3; H1F4; H1F5; H1FNT; H1FOO; H1FT; H1FV; H1FX; H1t; H1T2; H1X; HANP1; His1; HisC; HIST1; HIST1H1A; HIST1H1B; HIST1H1C; HIST1H1D; HIST1H1E; HIST1H1T; Oocyte-specific histone H1; Testicular H1 histone

Positive Control

HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma. Human heart tissue lysate.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of human leukemia biopsy cells

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

~30kDa

Specificity & Comments

Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bioinformatics

  •  Entrez Gene ID: 3005
  •  Gene Symbol: H1
  •  SwissProt: Multiple
  •  Chromosome Location: 3q21-q22; 6q21-q22; 22q13.1
  •  Unigene: 226117, 97358
NBT00096