Home >> Antibodies >> Histone H1 (Pan Nuclear Marker)

Histone H1 (Pan Nuclear Marker)

Recombinant Mouse Monoclonal Antibody [Clone rHH1/8702]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Histone Recombinant Mouse Monoclonal Antibody (rHH1/8702). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human lymph node stained with Histone H1 Recombinant Mouse Monoclonal Antibody (rHH1/8702). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Histone Recombinant Mouse Monoclonal Antibody (rHH1/8702). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human lymph node stained with Histone H1 Recombinant Mouse Monoclonal Antibody (rHH1/8702). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

HANP1; His1; HisC; HIST1; HIST1H1A; HIST1H1B; HIST1H1C; HIST1H1D; HIST1H1E; HIST1H1T; Oocyte-specific histone H1; Testicular H1 histone

Positive Control

HeLa, A-431, LNCap or Jurkat cells. Human heart or breast carcinoma.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of human leukemia biopsy cells

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

~30kDa

Specificity & Comments

Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.