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Home >> Antibodies >> HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)

Mouse Monoclonal Antibody [Clone HIF1A/84]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Immunofluorescence Analysis of MeOH-fixed HeLa cells labeled with HIF1 alpha Mouse Monoclonal Antibody (HIF1A/84) followed bygoat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Product Details

Synonyms

ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Hypoxia inducible factor 1 alpha; Member of PAS superfamily 1 (MOP1); PAS domain-containing protein 8 (PASD8)

Positive Control

Cobalt chloride treated HeLa cells. Breast or bladder carcinomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human HIF1 alpha protein

Cellular Localization

Nuclear and cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

92-110kDa

Specificity & Comments

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304. 2. Wang GL and Semenza GL. 1993. J. Biol. Chem. 268:21513. 3. Wang GL and Semenza GL. 1995. J. Biol. Chem. 270:1230. 4. Galbraith MD, et al. 2013. Cell. 153:1327.

Bioinformatics

  •  Entrez Gene ID: 3091
  •  Gene Symbol: HIF1A
  •  SwissProt: Q16665
  •  Chromosome Location: 14q23.2
  •  Unigene: 597216
NBT00084