Flow Cytometric Analysis of human trypsinized MCF-7 cells using HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
SDS-PAGE Analysis Purified HER-2 Mouse Monoclonal Antibody (HRB2/776). Confirmation of Integrity and Purity of Antibody.
Immunofluorescent staining of MeOH-fixed MCF-7 cells with HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
Immunofluorescent staining of PFA-fixed MCF-7 cells with HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by goat anti-mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.