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Home >> Antibodies >> Heparan Sulfate Proteoglycan (Large) / Perlecan

Heparan Sulfate Proteoglycan (Large) / Perlecan

Rat Monoclonal Antibody [Clone A7L6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Heparan Sulfate Monoclonal Antibody (A7L6).

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SDS-PAGE Analysis Purified Heparan Sulfate Monoclonal Antibody (A7L6). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Heparan sulfate proteoglycan of basement membrane; HSPG; Hspg2; LG3 peptide; Perlecan; PLC; SJS1

Positive Control

Breast carcinomas, squamous cell carcinomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Murine EHS laminin preparation

Cellular Localization

Basement membrane

Species Reactivity

Human. Monkey. Cow. Pig. Mouse. Fish.

Host / Ig Isotype

Rat / IgG2a, kappa

Mol. Weight of Antigen

>400kDa

Specificity & Comments

This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Ljubimov et. al., Lab Invest, 1995; 72:461-473.
  2. Couchman et. al., Matrix, 1989; 9:311-321.
  3. Folkvord et. al., J Histochem Cytochem, 1989; 37:105-113.
  4. Horiguchi et. al., J Histochem Cytochem, 1989; 37:961-970.
  5. Ljubimov et. al., Int J Cancer, 1992; 50:562-566.
  6. Guelstein et. al., Int J Cancer, 1993; 53:269-277.

Bioinformatics

  •  Entrez Gene ID: 3339
  •  Gene Symbol: HSPG2
  •  SwissProt: P98160
  •  Chromosome Location: 1p36.1-p34
  •  Unigene: 562227
NBT00315