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Home >> Antibodies >> HA Tag

HA Tag

Mouse Monoclonal Antibody [Clone 16.43]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Western Blot Analysis of HA-Tag recombinant protein using HA-Tag Mouse Monoclonal Antibody (16.43).

Product Details

Synonyms

HA epitope tag; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain

Positive Control

Any protein with a C-terminal HA tag.

Known Applications & Suggested Dilutions

ELISA, IF, Western Blot, IP, Flow Cyt, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

C-terminal influenza virus hemagglutinin (HA) tag, YPYDVPDYA

Cellular Localization

HA Tag in cytoplasm

Species Reactivity

Virus (influenza)

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Biehs R., et al. Mol Cell 65:671-684.e5 (2017).

Bioinformatics

NBT02543