2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> GRP94 / HSP90B1 (Endoplasmic Reticulum Marker)

GRP94 / HSP90B1 (Endoplasmic Reticulum Marker)

Rat Monoclonal Antibody [Clone SPM249]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Immunofluorescence Analysis of HePG2 cells labeling GRP94 with GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot.(Red).

img

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).

img

Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Glucose Regulated Protein 94; 94kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)

Positive Control

HePG2. U20S cells. About 50% of breast tumors express grp94 protein.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Purified glucose regulated protein 94 (grp94) from chicken oviducts

Cellular Localization

Cytoplasmic and nuclear

Species Reactivity

Human. Monkey. Horse. Cow. Sheep. Pig. Rabbit. Dog. Rat. Mouse. Hamster. Guinea Pig. Chicken. Xenopus laevis.

Host / Ig Isotype

Rat / IgG2a, kappa

Mol. Weight of Antigen

94kDa

Specificity & Comments

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987).
  2. Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990).

Bioinformatics

  •  Entrez Gene ID: 7184
  •  Gene Symbol: HSP90B1
  •  SwissProt: P14625
  •  Chromosome Location: 12q23.3
  •  Unigene: 192374
NBT00940