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Home >> Antibodies >> Golgi Complex (Marker for Human Cells)

Golgi Complex (Marker for Human Cells)

Mouse Monoclonal Antibody [Clone AE-6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Pancreas stained with Golgi Monoclonal Antibody (AE-6).

Product Details

Synonyms

Golgi Complex; Golgi apparatus; Golgi zone

Positive Control

HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, ICC, IHC-P         More Details

Flow Cytometry (0.5-1.0ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunocytochemistry (Acetone or paraformaldehyde fixed) (1-2ug/ml for 30 min)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

SU-DHL-1 large cell lymphoma cells

Cellular Localization

Golgi complex in cytoplasm

Species Reactivity

Human. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Not Known

Specificity & Comments

This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Immunofluorescence Analysis of HeLa cells labeling Fascin with Fascin-1 Mouse Monoclonal Antibody (FSCN1/417)-CF640 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red)

Bioinformatics

NBT00868