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Home >> Antibodies >> Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker)

Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker)

Mouse Monoclonal Antibody [Clone SPM595]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to CF555

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF555

Price (USD)

429.00

Product Details

Synonyms

DGSX; Glypican proteoglycan 3; GPC3; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI-5; SDYS; Secreted glypican-3; SGBS1

Positive Control

293T cells. Hepatocellular carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A recombinant fragment containing amino acids 511-580 of human glypican-3

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

67kDa

Specificity & Comments

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

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Key References

  1. Yan, B., et al. 2011. Ann. Diagn. Pathol. 15: 162-169.

Bioinformatics

  •  Entrez Gene ID: 2719
  •  Gene Symbol: GPC3
  •  SwissProt: P51654
  •  Chromosome Location: Xq26.2
  •  Unigene: 644108
NBT00862