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Home >> Antibodies >> Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker)

Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker)

Mouse Monoclonal Antibody [Clone 1G12]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma (HCC) stained with Glypican-3 Monoclonal Antibody (1G12)

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SDS-PAGE of Purified Glypican-3 Monoclonal Antibody (1G12). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of HePG2 cell lysate using Glypican-3 Monoclonal Antibody (1G12)

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Immunofluorescence Analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with Glypican-3 Monoclonal Antibody (1G12) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

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Flow Cytometric Analysis of MeOH-fixed HepG2 cells using Glypican-3 Monoclonal Antibody (1G12) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

DGSX; Glypican proteoglycan 3; GPC3; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI-5; SDYS; Secreted glypican-3; SGBS1

Positive Control

HepG2, 293T cells. Hepatocellular carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IF, IHC-P         More Details

Western Blot (1-2ug/ml)Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A recombinant fragment containing amino acids 511-580 of human glypican-3

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

67kDa

Specificity & Comments

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm's tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm's tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yan, B., et al. 2011. Expression and clinicopathologic significance of glypican 3 in hepatocellular carcinoma. Ann. Diagn. Pathol. 15: 162-169.

Bioinformatics

  •  Entrez Gene ID: 2719
  •  Gene Symbol: GPC3
  •  SwissProt: P51654
  •  Chromosome Location: Xq26.2
  •  Unigene: 644108
NBT00862