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Home >> Antibodies >> GFAP (Astrocyte & Neural Stem Cell Marker)

GFAP (Astrocyte & Neural Stem Cell Marker)

Mouse Monoclonal Antibody [Clone GA-5 + ASTRO/789]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Monoclonal Antibody (GA-5 + ASTRO/789).

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Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP Monoclonal Antibody (GA-5 + ASTRO/789).

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Formalin-fixed, paraffin-embedded Rat Cerebellum stained with GFAP Monoclonal Antibody (GA-5 + ASTRO/789).

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Western Blot Analysis of human brain tissue lysate using GFAP MAb (GA-5 + ASTRO/789).

Product Details

Synonyms

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Positive Control

T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

GFAP isolated from pig spinal cord (GA-5); Recombinant GFAP protein (ASTRO/789)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat. Cow. Pig. Rabbit. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~50kDa

Specificity & Comments

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. McLendon, R.E. and Bigner, D.D. 1994. Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations. Brain Pathol. 4: 221-228.

Bioinformatics

  •  Entrez Gene ID: 2670
  •  Gene Symbol: GFAP
  •  SwissProt: P14136
  •  Chromosome Location: 17q21.31
  •  Unigene: 514227
NBT00898